Ex vivo fluid secretion (Ramsay) assay
Abstract Insects have evolved a variety of neurohormones that enable them to maintain nutrient and osmotic homeostasis. Here, we characterized the ion transport peptide (ITP) signaling system in Drosophila. The Drosophila ITP gene can generate three different peptide isoforms: ITP amidated (ITPa) and two ITP-like (ITPL1 and ITPL2) isoforms. We comprehensively characterized the expression of all three ITP […]
Identification of Gyc76C as a putative ITP receptor
Abstract Insects have evolved a variety of neurohormones that enable them to maintain nutrient and osmotic homeostasis. Here, we characterized the ion transport peptide (ITP) signaling system in Drosophila. The Drosophila ITP gene can generate three different peptide isoforms: ITP amidated (ITPa) and two ITP-like (ITPL1 and ITPL2) isoforms. We comprehensively characterized the expression of all three ITP […]
Gyc76C characterization in HEK293T cells
Abstract Insects have evolved a variety of neurohormones that enable them to maintain nutrient and osmotic homeostasis. Here, we characterized the ion transport peptide (ITP) signaling system in Drosophila. The Drosophila ITP gene can generate three different peptide isoforms: ITP amidated (ITPa) and two ITP-like (ITPL1 and ITPL2) isoforms. We comprehensively characterized the expression of all three ITP […]